COURSES:
ENTOM 7006: Advanced Inset Pest Management
During the last decade, there have been numerous scientific and technological advances in insect pest management, such as use of the internet systems, global positioning systems (GPS) and geographical information systems (GIS), modeling, biotechnology, host plant resistance, biological control, as well as many discoveries of environmentally benign novel pesticide chemistries.
This course provides students with cutting edge information and knowledge related to the development and implementation of modern insect pest management tactics and strategies. The course will begin with an overview of pest management history and the ecological basis of insect pest management. Subsequent lectures will emphasize advances in major insect pest management tactics including cultural control, biological control, host plant resistance, and chemical control. Several key components of insect pest management, such as insect sampling, economic injury level (EIL), economic threshold (ET), and an introduction of system analysis and its applications in insect pest management will then be presented to underscore the quantitative aspects of insect pest management programs. Several lectures at the end of the course will focus on two novel technologies (biotechnology and GPS/GIS) and their applications in insect pest management. No comparable course exists at present at LSU.
ENTOM 7008: U.S. Agriculture and Major Agricultural Arthropod Pests
RESEARCH:
Major areas of research interest
Biology and management of corn, small grains, and stored-product insects
Efficacy of transgenic Bt plants
Bt resistance monitoring and management
Current research projects
Enhancing sustainable use of transgenic Bt-corn through resistance management(USDA-AFRI, Industries)
Characterizing Bacillus Thuringiensis resistance in insects (Louisiana Board of Regents, USDA ARS, and Industries).
Evaluation of novel Bt proteins and new Bt corn for management of corn borers (Industries)
Evaluation of novel insecticides for control of corn and sorghum insect pests (Industries)
PEOPLE
Fei Yang: PhD student (2011-): e-mail:fyang@agcenter.lsu.edu
Ying Niu: MS student (2011-): e-mail:yniu@agcenter.lsu.edu
Vikash Dangal: MS student (2012-): e-mail:vdanga1@tigera.lsu.edu
Xiaoyi Wu: PhD student (2004-2008):graduated in 2008 e-mail:Xiaoyiwu.cn1@gmail.com
Yunlong Yang: PhD student (2006-2011):graduated in 2011 e-mail: yangyunlong@neo.tamu.edu
Sindani D. Wangila: MS student (2010-2012):graduated in 2012 e-mail: sindanidavid@yahoo.com
Mukti N. Ghimire: Research Associate (2007-2011)
Bison Yue: Visiting Professor (2007):e-mail: bsyue@yahoo.com
Liping Zhang: Visiting Scholar (2010-2012): e-mail: liping2006@126.com
Yaoyu Bai: Visiting Scholar (2010-2011): e-mail: yybai2001@yahoo.com.cn
SELECTED PUBLICATIONS:
Book
Chen, J., X. Cheng, J. Cheng, and F. Huang (eds). 1990. Integrated Pest Management. Agricultural Press. Beijing, China. 270 pp.
Book chapters
Huang, F., B.R. Leonard, X. Wu, and M.N. Ghimire. 2011. Risk assessment of sugarcane borer resistance to transgenic maize expressing Bacillus thuringiensis proteins. pp. 89-103. In “Genetically Engineered Crops: Biotechnology, Biosafety and Benefits”, ed by L. M. Grover Nova Science Publishers, Hauppauge, NY.
Huang, F. 2010. Resistance to transgenic Bacillus thuringiensis crops in target insect pests: current status and prospect.Pp. 179-192.In “Advances in Entomological Research: From Molecular Biology to Pest Management”, eds by T.X. Liu and L. Kang, Higher Education Press, Beijing, China.
Huang, F.,and B.R. Leonard. 2008. Detection and monitoring of Bacillus thuringiensis resistance alleles in the sugarcane borer, Diatraea saccharalis (F.). pp 1-17. In “Genetically Modified Plants: New Research Trends”, (eds) by T.V. Wolf and J. P. Koch. Nova Science Publishers, Hauppauge, NY.
Huang, F. 2005. Effects of transgenic Bt-plants on non-target organisms. In “Entomological Research: Progress and Prospect”, (eds) by T.X. Liu and L. Kang. Science Press. Beijing, China. pp 318-335.
Huang, F. and B. Subramanyam. 2003. Effects of ultrasound on Indianmeal moth reproduction. In “Advances in Stored Product Protection”, (eds) by P. F. Credland, D.M. Armitage, C.H. Bell, P.M. Cogan and E. Highley. Proceedings of the 8th International Working Conference on Stored Product Protection, July 22-26, 2002, York, UK. pp 852-857.
Refereed Publications
David S. Wangila, D.S., B.R. Leonard, Y. Bai, G.P. Head, and F. Huang. 2012. Larval survival and plant injury of Cry1Ab-susceptible, -resistant, and -heterozygous genotypes of the sugarcane borer on transgenic corn containing single or pyramided Bt genes. Crop Prot. In press.
Yang, Y., Y.-C. Zhu, Y-C., J. Ottea1, C. Husseneder, B. R. Leonard, C. Abel, R, Luttrell, and F. Huang. 2012. Characterization and mRNA quantitative analyses of cDNAs encoding three trypsin- and chymotrypsin-like proteinases in Cry1Ab-susceptible and -resistant strains of sugarcane borer, Diatraea saccharalis. Ins. Sci. DOI 10.1111/j.1744-7917.2012.01514.x.
Huang, F., M.N. Ghimire, B. R. Leonard, Y-C. Zhu, and G. Head.2012. Susceptibility of field populations of sugarcane borer from non-Bt and Bt maize plants to five individual Cry toxins. Ins. Sci. 19:570-578.
Bai, Y., R.Yan, G. Ye, F. Huang, D.S. Wangila, J. Wang, and J. Cheng. 2012. Field response of aboveground non-target arthropod community to transgenic Bt-Cry1Ab rice plant residues in postharvest seasons. Transgenic Res. 21:1023–1032.
Huang, F., M.N. Ghimire, B.R. Leonard, C.D. Daves, R. Levy, J. Baldwin. 2012. Extended monitoring of resistance to Bacillus thuringiensis Cry1Ab maize in Diatraea saccharalis (Lepidoptera: Crambidae). GM Crop. Food: Biotech. Agri. Food Chain. 3:245-254.
Guo, Z., Y.C. Zhu, F. Huang, R. Luttrell, and R. Leonard. 2012. Microarray analysis of global gene regulation in the Cry1Ab-resistance and -susceptible strains of Diatraea saccharalis. Pest Manag. Sci. 2012; 68: 718–730.
Tabashnik, B.E., F. Huang, M. N. Ghimire, B. R. Leonard, B. D. Siegfried, M. Rangasamy, Y. Yang, Y. Wu, L. J. Gahan, D.G. Heckel, A. Bravo, and M. Soberón. 2011. Efficacy of genetically modified Bt toxins against insects with different mechanisms of resistance. Nature Biotechnol. 19:1128-1131.
Bai, Y., R. Yan, X. Ke, G. Ye, F. Huang, Y. Luo, J. Cheng. 2011. Effects of transgenic rice expressing Bacillus thuringiensis Cry1Ab protein on growth, reproduction, and superoxide dismutase activity of Folsomia candida (Collembola: Isotomidae). J. Econ. Entom. 104: 1892-1899.
Yang, Y., Y.C. Zhu, J. Ottea, C. Husseneder, B.R.Leonard, C. Abel, R.L. Lutrell, and F. Huang. 2011. Down regulation of a gene for cadherin, but not alkaline phosphatase, associated with Cry1Ab resistance in the sugarcane borer Diatraea saccharalis. PLoS ONE 6(10): e25783. doi:10.1371/journal.pone.0025783.
Huang, F., D.A. Andow, and L.L. Buschman. 2011. Success of the high dose/refuge resistance management strategy after 15 years of Bt crop use in North America. Entom. Exp. App. 140: 1-16.
Tan, S.Y., B.F. Cayabyab, E.P. Alcantara, Y. Ibrahim, F. Huang, E. Blankenship, and B.D. Siegfried. 2011. Comparative susceptibility of Ostrinia furnacalis, Ostrinia nubilalis and Diatraea saccharalis (Lepidoptera: Crambidae) to Bacillus thuringiensis Cry1 toxins. Crop Protect. 30: 1184-1189.
Huang, F., M.N. Ghimire, B.R. Leonard, J. Wang, C. Daves, R. Levy, D. Cook, G.P. Head, Y. Yang, J. Temple, and Rob Ferguson. 2011, F2 screening for resistance to pyramided Bacillus thuringiensis maize in Louisiana and Mississippi populations of Diatraea saccharalis (Lepidoptera: Crambidae). Pest Manag. Sci. 67:1269-1276.
Hardke, J.T., B.R Leonard, F. Huang, and R.E. Jackson. 2011. Damage and survivorship of fall armyworm (Lepidoptera: Noctuidae) on transgenic field corn expressing Bacillus thuringiensis Cry proteins. Crop Protect. 30:168-172.
Ghimire, M.N, F. Huang, R.B. Leonard, G. P. Head, and Y. Yang. 2011. Susceptibility of Cry1Ab-susceptible and -resistant sugarcane borer to transgenic corn plants containing single or pyramided Bacillus thuringiensis genes. Crop Protect. 30: 74-81.
Li, W., X. Zhang, Z. Fan, B. Yue, F. Huang, E. King, and J. Ran. 2011. Structural characteristics and phylogenetic analysis of the mitochondrial genome of the sugarcane borer, Diatraea saccharalis (Lepidoptera: Crambidae). DNA and Cell Biol. 30:3-8.
Yan X.H, H. Liu, J.J. Wang, Z.M. Zhao, F. Huang, and D.F. Cheng. 2010. Population forecasting model of brown planthopper and whitebacked planthopper (Homoptera: Delphacidae) based on Markov chain theory. Environ. Entomol. 39: 1737-1743.
Yang, Y., Y.C. Zhu, J. Ottea, C. Husseneder, B.R.Leonard and F. Huang. 2010. Molecular characterization and RNA interference of three midgut aminopeptidase N isozymes from Bacillus thuringiensis-susceptible and -resistant strains of sugarcane borer, Diatraea saccharalis. Ins. Biochem. Mol. Biol. 40: 592-603.
Bai, Y.Y., R. H. Yan, G. Y. Ye, F. Huang, and J. A. Cheng. 2010. Effects of Transgenic Rice Expressing Bacillus thuringiensis Cry1Ab Protein on Ground-Dwelling Collembolan Community in Postharvest Seasons. Environ. Entomol.39:243-251.
Liu, F., Z. Xu, Y. C. Zhu, F. Huang, Y. Wang, C. Gao, W. Zhou, and J. Shen. 2010. Evidence of field-evolved resistance to Cry1Ac-expressing Bt cotton in Helicoverpa armigera (Lepidoptera: Noctuidae) in Northern China. Pest Manage. Sci.66: 155-164
Wu, X., F. Huang, and B.R. Leonard, and Mukti Ghimire. 2009. Growth and development of Bacillus thuringiensis Cry1Ab-susceptible and -resistant sugarcane borer on diet and conventional corn plants. Entomol. Exp. Appl.133: 199–207.
Huang, F., Bh. Subramanyam, and R.Roesli. 2009. Comparative susceptibility of Liposcelis bostrychophila Badonnel and L. decolor (Pearman) (Psocoptera: Liposcelididae) to spinosad on wheat. Biopestic. Int. 5: 106-113.
Huang, F., R. Parker, B.R. Leonard, Y. Yong, and Jin Liu. 2009. Frequency of resistance alleles to Bacillus thuringiensis-corn in Texas populations of the sugarcane borer, Diatraea saccharalis (F.) (Lepidoptera: Crambidae). Crop Protect. 28: 174-180.
Wu, X., F. Huang, B.R. Leonard, and J. Ottea. 2009. Inheritance of resistance to Bacillus thuringiensis Cry1Ab protein in the sugarcane borer (Lepidoptera: Crambidae. J. Invertebr. Pathol. 102: 44–49.
Xu, Z., F. Liu, J. Chen, F. Huang, D.A. Andow, J. Shen, and Y.C. Zhu. 2009. Using F2 screen to monitor resistance allele frequency to Bt cotton in field populations of Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae). Pest Manag. Sci.. 65: 391-397.
Wu, X., B.R. Leonard, Y-C Zhu, C.A. Abel, G.P. Head, and F. Huang. 2009. Susceptibility of Cry1Ab-resistant and -susceptible sugarcane borer (Lepidoptera: Crambidae) to four Bacillus thuringiensis toxins. J. Invert. Pathol. 100:29-34.
Akbar, W., J. A. Ottea, J. M. Beuzelin, T.E. Reagan, and F. Huang. 2008. Selection and life history traits of tebufenozide-resistant sugarcane borer (Lepidoptera: Crambidae). J. Econ. Entomol. 101: 1903-1910.
Huang, F., B.R. Leonard, S.H. Moore, D.R. Cook, J. Baldwin, K. Tindall, and D. Lee. 2008. Allele frequency of resistance to Bacillus thuringiensis Cry1Ab corn in Louisiana populations of sugarcane borer (Lepidoptera: Crambidae). J. Econ. Entomol. 101: 492-498.
Huang, F., B.R. Leonard, S.H. Moore, B. Yue, R. Parker, T. Reagan, M. Stout, D.R. Cook, W. Akbar, C. Chilcutt, W. White, D. Lee, and S. Biles. 2008. Geographical susceptibility of Louisiana and Texas populations of sugarcane borer, Diatraea saccharalis (F.) (Lepidoptera: Crambidae) to Bacillus thuringiensis Cry1Ab protein. Crop Protect. 27: 799-806.
Yue, B., F. Huang, B.R. Leonard, S.H. Moore, R. Parker, D.A. Andow, D.R. Cook, K. Emfinger, and D.R. Lee. 2008.Verifying an F1 screen for identification and quantification of rare Bacillus thuringiensis resistance alleles in field populations of sugarcane borer(Lepidoptera: Crambidae)Entomol. Exp. App. 129: 172-180.
Huang, F., B.R. Leonard, and D.A. Andow. 2007. F2 screen for resistance to a Bacillus thuringiensis-maize hybrid in sugarcane borer (Lepidoptera: Crambidae). Bull. Entomol. Res. 97: 437-444.
Li, H., L.L. Buschman, F. Huang, K.Y. Zhu, B. Bonning, and B. Oppert. 2007. Dipel-selected Ostrinia nubilalis larvae are not resistant to transgenic corn expressing Bacillus thuringiensis Cry1Ab. J. Econ. Entomol. 100: 1862-1870.
Huang F., B.R. Leonard, D.R. Cook, D.R. Lee, D.A. Andow, J.L. Baldwin, K.V. Tindall, and X. Wu. 2007. Frequency of alleles conferring resistance to Bacillus thuringiensis maize in Louisiana populations of southwestern corn borer (Lepidoptera: Crambidae). Entomol. Exp. Appl. 122: 53 - 58.
Wu, X., F. Huang, B.R. Leonard, and S.H. Moore. 2007. Evaluation of transgenic Bacillus thuringiensis corn hybrids against Cry1Ab-susceptible and -resistant sugarcane borer (Lepidoptera: Crambidae). J. Econ. Entomol. 100: 1880-1886.
Huang, F. B.R. Leonard, and X. Wu. 2007. Resistance of sugarcane borer to Bacillus thuringiensis Cry1Ab toxin. Entomol. Exp. Appl. 124: 117-123.
Huang, F., B.R. Leonard, and D.A. Andow. 2007. Sugarcane borer resistance to transgenic Bacillus thuringiensis-maize. J. Econ. Entomol.100: 164-171.
p align="justify">Liu, C.Z., S.R. Zhou, L. Yan and F. Huang. 2007. Competition among the adults of three grasshoppers (Orthop: Acrididae) on an alpine grassland. J. App. Entomol.131: 153-159.
Huang, F., Bh. Subramanyam, and X. Hou. 2007. Efficacy of spinosad against eight stored-product insect species on hard white winter wheat. Biopestic. Int. 3: 117-125.
Huang, F. and Bh. Subramanyam. 2007. Effectiveness of spinosad against seven major stored-grain insects on corn. Insect Sci. 14:225-230.
Li, H., L.L. Buschman, K.Y. Zhu, F. Huang, and B. Oppert. 2007. Resistance to Bacillus thuringiensis endotoxins in the European corn borer (Ostrinia nubilalis). Biopestic. Int. 3: 96-107.
Huang, F., B.R. Leonard, and R.H. Gable. 2006. Comparative susceptibility of European corn borer, southwestern corn borer, and sugarcane borer (Lepidoptera: Crambidae) to Cry1Ab protein in a commercial Bt-corn hybrid. J. Econ. Entomol. 99: 194-202.
Huang, F. 2006. Detection and monitoring of insect resistance to transgenic Bt crops. Insect Science 13: 73-84.
Huang, F. and Bh. Subramanyam. 2006. Lack of repellency of three commercial ultrasonic devices to the German cockroach (Blattodea: Blattellidae). Insect Sci. 13: 61-66.
Li, H, B. Oppert, R.A. Higgins, F. Huang, L.L. Buschman, and K.Y. Zhu. 2005. Susceptibility of Dipel-resistant and -susceptible Ostrinia nubilalis (Lepidoptera: Crambidae) to individual Bacillus thuringiensis protoxins. J. Econ. Entomol. 98: 1333-1340. (Huang’s contribution: establishment of insect colonies, conducting insect bioassays, data analysis, and manuscript writing).
Li H., B. Oppert, R.A. Higgins, F. Huang, L.L. Buschman, J.R. Gao, and K.Y.Zhu. 2005. Characterization of cDNAs encoding three trypsin-like proteinases and quantitative analysis of mRNA in Bt-resistant and -susceptible strains of Ostrinia nubilalis. Insect Biochem. Mol. Biol. 35: 847-860.
Huang, F. and Bh. Subramanyam. 2005. Management of five stored-product insects in wheat with pirimiphos-methyl and pirimiphos-methyl plus synergized pyrethrins. Pest Manag. Sci. 61: 356-362.
Huang, F., L.L. Buschman, and R.A. Higgins. 2005. Larval survival and development of susceptible and resistant Ostrinia nubilalis (Lepidoptera: Pyralidae) on diet containing Bacillus thuringiensis. Agri. Forest. Entomol. 7: 45-52.
Huang, F. and Bh. Subramanyam. 2004. Behavioral and reproductive effects of ultrasound on Plodia interpunctella (Hübner). Entomol. Exp. Appl. 113: 157-164.
Li, H., J. Gonzalez, B. Oppert, J. Ferre, R.A. Higgins, L.L. Buschman, G.A. Radke, K.Y.Zhu, and F. Huang. 2004. Binding analyses of Cry1Ab and Cry1Ac with membrane vesicles from Bacillus thuringiensis-resistant and –susceptible Ostrinia nubilalis. Biochem. Biophysi. Res. Comm. 323: 52-57.
Li, H., B. Oppert, R.A. Higgins, F. Huang, K.Y. Zhu, and L.L. Buschman.2004. Comparative analysis of proteinase activities of Bacillus thuringiensis-resistant and -susceptible Ostrinia nubilalis (Lepidoptera: Crambidae). Insect Biochem. Mol. Biol. 34: 753-762.
Huang, F., Bh. Subramanyam, and M.D. Toews. 2004. Susceptibility of laboratory and field strains of four stored-product insect species to spinosad. J. Econ. Entomol. 97: 2154-2159.
Huang, F. and Bh. Subramanyam. 2003. Responses of Corcya cephalonica (Stainton) to pirimiphos-methyl, spinosad, and combination of pirimiphos-methyl and synergized pyrethrins. Pest Manag. Sci. 60: 191-198.
Huang, F., Bh. Subramanyam, and R. Taylor. 2003. Ultrasound affects spermatophore transfer,larval numbers, and larval weight of Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae). J. Stored Prod. Res. 39: 413-422.
Huang, F., and Bh. Subramanyam. 2003. Effects of delayed mating on reproductive performance of Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae). J. Stored Prod. Res. 39: 53-63.
Li, H., B. Oppert, K.Y. Zhu, R.A. Higgins, F. Huang, and L.L. Buschman. 2003. Transgenic plants expressing Bacillus thuringiensis delta-endotoxins. Entomol. Sinica10: 155-166.
Huang, F., L.L. Buschman, R.A. Higgins, and H. Li. 2002. Survival of Kansas Dipel-resistant European corn borer (Lepidoptera: Crambidae) on Bt and non-Bt corn hybrids. J. Econ. Entomol. 95: 614-621.
Huang, F., Bh. Subramanyam, and J. Clark. 2002. Laboratory and field trials with commercial ultrasonic devices against three ant species (Hymenoptera: Formicidae). J. Agric. Urban Entomol. 19: 25-28.
Huang, F., L.L. Buschman, and R.A. Higgins. 2001. Larval feeding behavior of Dipel-resistant and susceptible Ostrinia nubilalis on diet containing Bacillus thuringiensis (Dipel ESTM). Entomol. Exp. Appl. 98: 141─148.
Huang, F., K.Y. Zhu, L.L. Buschman, R.A. Higgins, and B. Oppert. 1999. Comparison of midgut proteinases in Bacillus thuringiensis-susceptible and -resistant European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae). Pest. Biochem. Physiol. 65: 132-139.
Huang, F., R.A. Higgins, and L.L. Buschman. 1999. Heritability and stability of resistance to Bacillus thuringiensis in European corn borer (Lepidoptera: Pyralidae). Bull. Entomol. Res. 89: 449-454.
Huang, F., L.L. Buschman, and R.A. Higgins. 1999. Susceptibility of different instars of European corn borer (Lepidoptera: Crambidae), to Bacillus thuringiensis. J. Econ. Entomol. 92: 547-550.
Huang, F., L.L. Buschman, R.A. Higgins, and W.H. McGaughey. 1999. Inheritance of resistance to Bacillus thuringiensis toxin (Dipel ESTM)in European corn borer. Science (Wash.). 284: 965-967.
Huang, F., R.A. Higgins, and L.L. Buschman. 1999. Transgenic Bt plants: successes, challenges, and strategies. Pestology 23: 2-29.
Huang, F., R.A. Higgins, and L.L. Buschman. 1997. Baseline susceptibility and changes in susceptibility to Bacillus thuringiensis subsp. kurstaki under selection pressure in European corn borer (Lepidoptera: Pyralidae). J.Econ. Entomol. 90: 1137-1143.
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